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95
Thermo Fisher sodium bicarbonate
Growth kinetics of VERO cells expanded in OptiVERO in 3D culture. VERO cells were adapted to each tested medium for at least three passages in T‐flasks before microcarrier runs. Cells were seeded at an initial density of 10,000 cells/cm 2 at Day 0 in 150 ml spinners. At Day 2 postseeding, spinners were monitored every day for cell growth, culture glucose, and pH. When necessary, cultures were fed with 45% glucose and 7.5% sodium <t>bicarbonate</t> to maintain the culture glucose at 2 g/L and pH neutral. VERO cells cultured in OptiVERO readily attached to the microcarriers and demonstrated a significantly higher cell expansion than either EMEM‐10% FBS (***) or VP‐SFM ( ### ) as determined by one‐way ANOVA. Data are a representative run of at least four independent experiments. 3D, three‐dimensional; ANOVA, analysis of variance; EMEM, Eagle's minimum essential medium; FBS, fetal bovine serum
Sodium Bicarbonate, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Thermo Fisher j61360 ak sds solution
Growth kinetics of VERO cells expanded in OptiVERO in 3D culture. VERO cells were adapted to each tested medium for at least three passages in T‐flasks before microcarrier runs. Cells were seeded at an initial density of 10,000 cells/cm 2 at Day 0 in 150 ml spinners. At Day 2 postseeding, spinners were monitored every day for cell growth, culture glucose, and pH. When necessary, cultures were fed with 45% glucose and 7.5% sodium <t>bicarbonate</t> to maintain the culture glucose at 2 g/L and pH neutral. VERO cells cultured in OptiVERO readily attached to the microcarriers and demonstrated a significantly higher cell expansion than either EMEM‐10% FBS (***) or VP‐SFM ( ### ) as determined by one‐way ANOVA. Data are a representative run of at least four independent experiments. 3D, three‐dimensional; ANOVA, analysis of variance; EMEM, Eagle's minimum essential medium; FBS, fetal bovine serum
J61360 Ak Sds Solution, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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j61360 ak sds solution - by Bioz Stars, 2026-08
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Thermo Fisher hydroxyethyl 1 piperazineethanesulfonic acid hepes thermo fisher scienti c
Growth kinetics of VERO cells expanded in OptiVERO in 3D culture. VERO cells were adapted to each tested medium for at least three passages in T‐flasks before microcarrier runs. Cells were seeded at an initial density of 10,000 cells/cm 2 at Day 0 in 150 ml spinners. At Day 2 postseeding, spinners were monitored every day for cell growth, culture glucose, and pH. When necessary, cultures were fed with 45% glucose and 7.5% sodium <t>bicarbonate</t> to maintain the culture glucose at 2 g/L and pH neutral. VERO cells cultured in OptiVERO readily attached to the microcarriers and demonstrated a significantly higher cell expansion than either EMEM‐10% FBS (***) or VP‐SFM ( ### ) as determined by one‐way ANOVA. Data are a representative run of at least four independent experiments. 3D, three‐dimensional; ANOVA, analysis of variance; EMEM, Eagle's minimum essential medium; FBS, fetal bovine serum
Hydroxyethyl 1 Piperazineethanesulfonic Acid Hepes Thermo Fisher Scienti C, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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hydroxyethyl 1 piperazineethanesulfonic acid hepes thermo fisher scienti c - by Bioz Stars, 2026-08
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Thermo Fisher sodium borate buffer
Growth kinetics of VERO cells expanded in OptiVERO in 3D culture. VERO cells were adapted to each tested medium for at least three passages in T‐flasks before microcarrier runs. Cells were seeded at an initial density of 10,000 cells/cm 2 at Day 0 in 150 ml spinners. At Day 2 postseeding, spinners were monitored every day for cell growth, culture glucose, and pH. When necessary, cultures were fed with 45% glucose and 7.5% sodium <t>bicarbonate</t> to maintain the culture glucose at 2 g/L and pH neutral. VERO cells cultured in OptiVERO readily attached to the microcarriers and demonstrated a significantly higher cell expansion than either EMEM‐10% FBS (***) or VP‐SFM ( ### ) as determined by one‐way ANOVA. Data are a representative run of at least four independent experiments. 3D, three‐dimensional; ANOVA, analysis of variance; EMEM, Eagle's minimum essential medium; FBS, fetal bovine serum
Sodium Borate Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Thermo Fisher borate buffer
Growth kinetics of VERO cells expanded in OptiVERO in 3D culture. VERO cells were adapted to each tested medium for at least three passages in T‐flasks before microcarrier runs. Cells were seeded at an initial density of 10,000 cells/cm 2 at Day 0 in 150 ml spinners. At Day 2 postseeding, spinners were monitored every day for cell growth, culture glucose, and pH. When necessary, cultures were fed with 45% glucose and 7.5% sodium <t>bicarbonate</t> to maintain the culture glucose at 2 g/L and pH neutral. VERO cells cultured in OptiVERO readily attached to the microcarriers and demonstrated a significantly higher cell expansion than either EMEM‐10% FBS (***) or VP‐SFM ( ### ) as determined by one‐way ANOVA. Data are a representative run of at least four independent experiments. 3D, three‐dimensional; ANOVA, analysis of variance; EMEM, Eagle's minimum essential medium; FBS, fetal bovine serum
Borate Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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borate buffer - by Bioz Stars, 2026-08
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94
Thermo Fisher taps wash buffer
Growth kinetics of VERO cells expanded in OptiVERO in 3D culture. VERO cells were adapted to each tested medium for at least three passages in T‐flasks before microcarrier runs. Cells were seeded at an initial density of 10,000 cells/cm 2 at Day 0 in 150 ml spinners. At Day 2 postseeding, spinners were monitored every day for cell growth, culture glucose, and pH. When necessary, cultures were fed with 45% glucose and 7.5% sodium <t>bicarbonate</t> to maintain the culture glucose at 2 g/L and pH neutral. VERO cells cultured in OptiVERO readily attached to the microcarriers and demonstrated a significantly higher cell expansion than either EMEM‐10% FBS (***) or VP‐SFM ( ### ) as determined by one‐way ANOVA. Data are a representative run of at least four independent experiments. 3D, three‐dimensional; ANOVA, analysis of variance; EMEM, Eagle's minimum essential medium; FBS, fetal bovine serum
Taps Wash Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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taps wash buffer - by Bioz Stars, 2026-08
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Thermo Fisher bicine buffer
Growth kinetics of VERO cells expanded in OptiVERO in 3D culture. VERO cells were adapted to each tested medium for at least three passages in T‐flasks before microcarrier runs. Cells were seeded at an initial density of 10,000 cells/cm 2 at Day 0 in 150 ml spinners. At Day 2 postseeding, spinners were monitored every day for cell growth, culture glucose, and pH. When necessary, cultures were fed with 45% glucose and 7.5% sodium <t>bicarbonate</t> to maintain the culture glucose at 2 g/L and pH neutral. VERO cells cultured in OptiVERO readily attached to the microcarriers and demonstrated a significantly higher cell expansion than either EMEM‐10% FBS (***) or VP‐SFM ( ### ) as determined by one‐way ANOVA. Data are a representative run of at least four independent experiments. 3D, three‐dimensional; ANOVA, analysis of variance; EMEM, Eagle's minimum essential medium; FBS, fetal bovine serum
Bicine Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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bicine buffer - by Bioz Stars, 2026-08
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94
Thermo Fisher epps ph8 5
Growth kinetics of VERO cells expanded in OptiVERO in 3D culture. VERO cells were adapted to each tested medium for at least three passages in T‐flasks before microcarrier runs. Cells were seeded at an initial density of 10,000 cells/cm 2 at Day 0 in 150 ml spinners. At Day 2 postseeding, spinners were monitored every day for cell growth, culture glucose, and pH. When necessary, cultures were fed with 45% glucose and 7.5% sodium <t>bicarbonate</t> to maintain the culture glucose at 2 g/L and pH neutral. VERO cells cultured in OptiVERO readily attached to the microcarriers and demonstrated a significantly higher cell expansion than either EMEM‐10% FBS (***) or VP‐SFM ( ### ) as determined by one‐way ANOVA. Data are a representative run of at least four independent experiments. 3D, three‐dimensional; ANOVA, analysis of variance; EMEM, Eagle's minimum essential medium; FBS, fetal bovine serum
Epps Ph8 5, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Average 94 stars, based on 1 article reviews
epps ph8 5 - by Bioz Stars, 2026-08
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95
Thermo Fisher buffer
Growth kinetics of VERO cells expanded in OptiVERO in 3D culture. VERO cells were adapted to each tested medium for at least three passages in T‐flasks before microcarrier runs. Cells were seeded at an initial density of 10,000 cells/cm 2 at Day 0 in 150 ml spinners. At Day 2 postseeding, spinners were monitored every day for cell growth, culture glucose, and pH. When necessary, cultures were fed with 45% glucose and 7.5% sodium <t>bicarbonate</t> to maintain the culture glucose at 2 g/L and pH neutral. VERO cells cultured in OptiVERO readily attached to the microcarriers and demonstrated a significantly higher cell expansion than either EMEM‐10% FBS (***) or VP‐SFM ( ### ) as determined by one‐way ANOVA. Data are a representative run of at least four independent experiments. 3D, three‐dimensional; ANOVA, analysis of variance; EMEM, Eagle's minimum essential medium; FBS, fetal bovine serum
Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 95/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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buffer - by Bioz Stars, 2026-08
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94
Thermo Fisher j61038 ap
Growth kinetics of VERO cells expanded in OptiVERO in 3D culture. VERO cells were adapted to each tested medium for at least three passages in T‐flasks before microcarrier runs. Cells were seeded at an initial density of 10,000 cells/cm 2 at Day 0 in 150 ml spinners. At Day 2 postseeding, spinners were monitored every day for cell growth, culture glucose, and pH. When necessary, cultures were fed with 45% glucose and 7.5% sodium <t>bicarbonate</t> to maintain the culture glucose at 2 g/L and pH neutral. VERO cells cultured in OptiVERO readily attached to the microcarriers and demonstrated a significantly higher cell expansion than either EMEM‐10% FBS (***) or VP‐SFM ( ### ) as determined by one‐way ANOVA. Data are a representative run of at least four independent experiments. 3D, three‐dimensional; ANOVA, analysis of variance; EMEM, Eagle's minimum essential medium; FBS, fetal bovine serum
J61038 Ap, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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j61038 ap - by Bioz Stars, 2026-08
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93
Rockland Immunochemicals tris hcl ph 8 5
Growth kinetics of VERO cells expanded in OptiVERO in 3D culture. VERO cells were adapted to each tested medium for at least three passages in T‐flasks before microcarrier runs. Cells were seeded at an initial density of 10,000 cells/cm 2 at Day 0 in 150 ml spinners. At Day 2 postseeding, spinners were monitored every day for cell growth, culture glucose, and pH. When necessary, cultures were fed with 45% glucose and 7.5% sodium <t>bicarbonate</t> to maintain the culture glucose at 2 g/L and pH neutral. VERO cells cultured in OptiVERO readily attached to the microcarriers and demonstrated a significantly higher cell expansion than either EMEM‐10% FBS (***) or VP‐SFM ( ### ) as determined by one‐way ANOVA. Data are a representative run of at least four independent experiments. 3D, three‐dimensional; ANOVA, analysis of variance; EMEM, Eagle's minimum essential medium; FBS, fetal bovine serum
Tris Hcl Ph 8 5, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Thermo Fisher native tbe gels
Growth kinetics of VERO cells expanded in OptiVERO in 3D culture. VERO cells were adapted to each tested medium for at least three passages in T‐flasks before microcarrier runs. Cells were seeded at an initial density of 10,000 cells/cm 2 at Day 0 in 150 ml spinners. At Day 2 postseeding, spinners were monitored every day for cell growth, culture glucose, and pH. When necessary, cultures were fed with 45% glucose and 7.5% sodium <t>bicarbonate</t> to maintain the culture glucose at 2 g/L and pH neutral. VERO cells cultured in OptiVERO readily attached to the microcarriers and demonstrated a significantly higher cell expansion than either EMEM‐10% FBS (***) or VP‐SFM ( ### ) as determined by one‐way ANOVA. Data are a representative run of at least four independent experiments. 3D, three‐dimensional; ANOVA, analysis of variance; EMEM, Eagle's minimum essential medium; FBS, fetal bovine serum
Native Tbe Gels, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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Image Search Results


Growth kinetics of VERO cells expanded in OptiVERO in 3D culture. VERO cells were adapted to each tested medium for at least three passages in T‐flasks before microcarrier runs. Cells were seeded at an initial density of 10,000 cells/cm 2 at Day 0 in 150 ml spinners. At Day 2 postseeding, spinners were monitored every day for cell growth, culture glucose, and pH. When necessary, cultures were fed with 45% glucose and 7.5% sodium bicarbonate to maintain the culture glucose at 2 g/L and pH neutral. VERO cells cultured in OptiVERO readily attached to the microcarriers and demonstrated a significantly higher cell expansion than either EMEM‐10% FBS (***) or VP‐SFM ( ### ) as determined by one‐way ANOVA. Data are a representative run of at least four independent experiments. 3D, three‐dimensional; ANOVA, analysis of variance; EMEM, Eagle's minimum essential medium; FBS, fetal bovine serum

Journal: Biotechnology and Bioengineering

Article Title: Formulation and production of a blood‐free and chemically defined virus production media for VERO cells

doi: 10.1002/bit.27486

Figure Lengend Snippet: Growth kinetics of VERO cells expanded in OptiVERO in 3D culture. VERO cells were adapted to each tested medium for at least three passages in T‐flasks before microcarrier runs. Cells were seeded at an initial density of 10,000 cells/cm 2 at Day 0 in 150 ml spinners. At Day 2 postseeding, spinners were monitored every day for cell growth, culture glucose, and pH. When necessary, cultures were fed with 45% glucose and 7.5% sodium bicarbonate to maintain the culture glucose at 2 g/L and pH neutral. VERO cells cultured in OptiVERO readily attached to the microcarriers and demonstrated a significantly higher cell expansion than either EMEM‐10% FBS (***) or VP‐SFM ( ### ) as determined by one‐way ANOVA. Data are a representative run of at least four independent experiments. 3D, three‐dimensional; ANOVA, analysis of variance; EMEM, Eagle's minimum essential medium; FBS, fetal bovine serum

Article Snippet: Culture glucose was maintained at 2 g/L and neutral pH via the addition of a 45% glucose solution (Sigma‐Aldrich) and 7.5% sodium bicarbonate (Thermo Fisher Scientific).

Techniques: Cell Culture