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Image Search Results
Journal: Biotechnology and Bioengineering
Article Title: Formulation and production of a blood‐free and chemically defined virus production media for VERO cells
doi: 10.1002/bit.27486
Figure Lengend Snippet: Growth kinetics of VERO cells expanded in OptiVERO in 3D culture. VERO cells were adapted to each tested medium for at least three passages in T‐flasks before microcarrier runs. Cells were seeded at an initial density of 10,000 cells/cm 2 at Day 0 in 150 ml spinners. At Day 2 postseeding, spinners were monitored every day for cell growth, culture glucose, and pH. When necessary, cultures were fed with 45% glucose and 7.5% sodium bicarbonate to maintain the culture glucose at 2 g/L and pH neutral. VERO cells cultured in OptiVERO readily attached to the microcarriers and demonstrated a significantly higher cell expansion than either EMEM‐10% FBS (***) or VP‐SFM ( ### ) as determined by one‐way ANOVA. Data are a representative run of at least four independent experiments. 3D, three‐dimensional; ANOVA, analysis of variance; EMEM, Eagle's minimum essential medium; FBS, fetal bovine serum
Article Snippet: Culture glucose was maintained at 2 g/L and neutral pH via the addition of a 45% glucose solution (Sigma‐Aldrich) and 7.5%
Techniques: Cell Culture